Micro-computed tomography is an x-ray imaging technique introduced to bone and mineral research in the late 1980's by Layton and Feldkamp. Since then this technology has advanced greatly, moving from systems that occupied entire rooms and were limited to 50 µm nominal resolutions to desktop systems that can now acquire 1 micron and sub-micron level data. With this data, it is possible to quantitatively analyze the 3D structure of trabecular bone and cortical bone, which is otherwise only possible with histology. This imaging method is now a standard analysis in studies of aging, disease, tissue engineering, and therapeutics as the pertain to bone and mineral.
The UTHSCSA Orthopaedics Bone and Mineral High-Resolution 3D Imaging Facility has a Sky Scan 1172 desktop micro-computed tomography system. This system has an X-ray source with a range of energy between 40 and 100 kV and CCD camera with 1k, 2k, and 4k settings. Depending on the object size and other factors. Images with up to a 1 micron nominal resolution can be acquired. Murine data are typically collected with 10-12 µm isotropic voxels. Cone-beam scanning facilitates fast acquisition of large volumes of data. The non-destructive nature of µCT imaging allows the researcher to strengthen his/her research by using the same sample for histology or biomechanical testing after µCT imaging.
To advance the research of institutional and regional orthopaedic researchers and other disciplines interested in bone, theeth, and mineral.
UTHSCSA and regional investigators interested in µCT analyses should contact Dr. Robert Fajardo (firstname.lastname@example.org or 210-567-6176) or James Schmitz (email@example.com or 210-567-5155) to set up a meeting. Pilot data (a few samples) acquisition will, in most cases, be at no cost. After the data are acquired, we will provide a report and help you interpret your results. Collaborative projects are preferred; fee-for-service (no collaboration, not data interpretation) projects are accepted on a limited basis. Dr. Fajardo has expertise/experience working with a variety of animal models and in a variety of applications including comparative anatomy, bone tissue engineering, disease and therapeutic intervention. Baseline rates (based on typical mouse analyses), including scan and morphometric analysis for academic collaborators are: $160 per specimen (1k camera), $180 per specimen (2K camera), and $220 per specimen (4k camera). Rates may vary according to project protocol. The pilot data phase will be used to establish a more appropriate per specimen cost for your project.